INSTITUTIONAL BIOSAFETY COMMITTEE

Laboratory Inspection Notice

Prior to initiating a BL2 or above study, all facilities that will be used for the study, including the laboratory and any shared facilities, must be inspected by the Institutional Biosafety Committee Biosafety Officer. Final approval will be granted once the laboratory meets the required laboratory standards.

To facilitate inspection of the laboratory, please note the following:

1.	All laboratory facilities for new BL2 or above studies must be inspected by the Biosafety Officer, whether  or not the facility was previously inspected.  
2.	The principal investigator and all staff involved in the project must be present during the inspection.  If this is not possible on the scheduled date, the inspection will be rescheduled accordingly.
3.	Prior to the inspection, please provide a summary of the experimental procedures to be used during experiments conducted with the BL2 agent.
4.	In the case of amendments from previously approved studies, any additions or changes in the host vector system will require Committee review and could result in a Committee requirement for a laboratory inspection.

Enclosed is a copy of special practices, as described by CDC-NIH, that must be followed when BL2 agents are being used.

    1.	A list of all viral work done in your laboratory is required, including:
  	a.  cell lines containing latent or defective viruses
	b.  virus producing cell lines
     	c.  purified viral stocks

    2.	If common equipment areas are used in these experiments, a list of any BL2 agents used.  

    3.	Included is a copy of the "Laboratory Biosafety Survey - BL2" form that will be filled out by the Biosafety Officer during the laboratory inspection.

Please forward the requested information with your application for BL2 level work to Research and Sponsored Programs.  After we review this information, the Biosafety Officer will contact you directly to schedule the inspection. 

If you have any questions concerning the laboratory inspection, please contact Research Compliance Administration
(274-8289) or James Klenner, Biosafety Officer (274-2830).

LABORATORY BIOSAFETY SURVEY - BL2

Principal Investigator	 	Department 	

Laboratory Building/Room No. _____________________________      Date____________________________________

Biological Agent  	

Health Concern 	

Surveyed By 	

Yes      No         Principal Investigator and research staff involved in the project were present for the inspection.

Yes	No	Puncture-resistant containers are available for disposal of contaminated sharps.

Yes	No	Durable leak proof containers are available for disposal of all non-sharp contaminated materials.

Yes	No	Laboratory coats, gowns, smocks, or uniforms are available and have a place for storage in the laboratory when not in use.

Yes	No	Appropriate gloves are available for the work to be performed.

Yes	No	Access to the laboratory is limited by the Principal Investigator.

Yes	No	A hazard warning sign is posted outside of the laboratory entry stating the following:  infectious agent used, name and telephone number of responsible person, and special requirements for entering the laboratory.

Yes	No	A Biosafety Manual and specific standard operating procedures for the infectious agent are located in the laboratory and have been read by all personnel involved in these experiments.

Yes	No	Appropriate and certified biological safety cabinet(s) are available in the laboratory and have been certified within the last 12 months.

Yes	No	Centrifuges used in the open laboratory have sealed heads or centrifuge safety cups.

Yes	No	Vacuum lines are protected with a double-trap and in-line filter located between the last trap and vacuum source.

Yes	No	Each laboratory contains a sink for hand washing.

Yes	No	An emergency eyewash facility is readily available.

Yes	No	An autoclave for decontaminating infectious laboratory wastes is available.

Yes	No	Equipment and work surfaces are appropriately decontaminated

Sources:	Guidelines for Research Involving Recombinant DNA Molecules (NIH Guidelines), January, 2001 and 
		Biosafety in Microbiological and Biomedical Laboratories, U.S. Department of Health and Human Services, Public Health Service, Centers for Disease Control and National Institutes for Health, 4th Edition, 1999.